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Bone allografts are frequently used during orthopaedic trauma cases or other reconstructive procedures. Most allografts are processed and cleaned before use. Our goals were to determine if an improved cleaning procedure compromises the strength or osteoinductivity of a graft. We compared our improved cleaning procedure to our standard cleaning procedure on cortical bone allograft. The cleaning procedures are generally composed of a series of chemical steps with nonionic detergents, hydrogen peroxide, and alcohol under time mtf nudes temperature control, subjected to ultrasonic agitation.

We tested the compressive strength, impact strength, and shear strength following the standard and improved cleaning procedures. Osteoinductivity was tested in 4 groups, using the improved cleaning procedure with four different hydrogen peroxide cleaning times: 0, 1, 3, and 5 h.

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Osteoinductivity was evaluated in vivo, using a day implant in the hamstring muscle of mtf nudes athymic, nude mouse. demonstrated that osteoinductivity is maintained with cleaning in hydrogen peroxide for up to 1 h, and that compressive strength, impact strength, and shear strength were all unaffected by the improved cleaning procedure. The improved cleaning procedure therefore did not compromise the strength or osteoinductivity of cortical bone allografts in comparison to the standard procedure.

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Similar articles A novel application of high-dose 50kGy gamma irradiation for demineralized bone matrix: effects on fusion rate in a rat spinal fusion model. Alanay A, et al. Spine J. Epub Aug 3. PMID: Mechanical strength of bone allografts subjected to chemical sterilization and other terminal processing methods.

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Mikhael MM, et al. J Biomech. Epub Aug Characterization of the mechanical properties of bovine cortical bone treated with a novel tissue sterilization process. Kemper N, et al. Cell Tissue Bank. Epub Jul Adjuvant therapies of bone graft around non-cemented experimental orthopedic implants stereological methods and experiments in dogs. Baas J. Acta Orthop Suppl. PMID: Review. Bone strength: the whole is greater than the sum of its parts. Davison KS, et al.

Semin Arthritis Rheum. Epub Jul 3. See all similar articles. Mtf nudes J, et al. Orthop Clin North Am. Epub Feb No abstract available. Evading the host response: Staphylococcus "hiding" in cortical bone canalicular system causes increased bacterial burden. Zoller SD, et al. Bone Res. Hydrogel-embedded nanocrystalline hydroxyapatite granules elastic blocks based on a cross-linked polyvinylpyrrolidone as bone grafting substitute in a rat tibia model. Dau M, et al. Int J Nanomedicine. Lu M, Hansen EN.

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Rashmi, et al. Vet World. Epub Feb 8. See all "Cited by" articles.

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Publication types Research Support, Non-U. Gov't Actions. MeSH terms Adult Actions. Animals Actions. Female Actions. Humans Actions. Male Actions. Mice Actions. Mice, Nude Actions. Middle Aged Actions. Research De Actions. Shear Strength Actions. Stress, Mechanical Actions. Substances Hydrogen Peroxide Actions.

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